Analysis Note

ControlUntreated and lambda phosphatase treated NIH/3T3 cell lysate

Application

Research Sub CategoryTranscription Factors

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242

Research CategoryEpigenetics & Nuclear Function

Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E1 (rat monoclonal) is a highly specific rat monoclonal antibody & that targets RNA Polymerase & has been tested in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-2′ CTD residue of RPB1.

Immunogen

Epitope: Phosphorylated Ser2 at the C-terminal domain (CTD)

Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).

Linkage

Replaces: 04-1571

Physical form

Unpurified rat monoclonal IgG1κ tissue culture supenatant with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in untreated and lambda phosphatase treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RNA Polymerase II subunit B1 (phospho CTD Ser-2) in untreated NIH/3T3 cell lysate.

Specificity

Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242

This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~220 kDa observed